Mycobacterium phage Liloa
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| Detailed Information for Phage Liloa | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kasie Stephens |
| Year Found | 2015 |
| Location Found | Cullowhee, NC USA |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.485086 N, 82.996489 W Map |
| Discovery Notes | My sample was taken from a compost sample in a WCU faculty member's backyard. Dr. Marth's sample had loads of phage in it. My average tail size of my phage was 0.204 um and the average head size was 0.062325 um. My DNA yield was 179.4 ng/um. |
| Naming Notes | Liloa stands for "Lil Orphan Annie". I acquired Liloa when my Phage Hunters instructor wanted me to further move his sample along. Since I was having a difficult time isolating my own phage I took Liloa under my wing and grew her up from the very beginning. She was an orphan and I so to speak "adopted" her. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | My phage produces small and medium sized lytic plaques that are characteristic of my phage. Plaques can generally be seen after 24 hours and my plaque sizes stayed consistent the entire duration of my Phage Hunters course. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2*10^11 pfu/ml |
| Date of SEA Lysate Titering | Oct 21, 2015 |
| Pitt Freezer Box# | 19 |
| Pitt Freezer Box Grid# | G7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |