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Streptomyces phage Lishka
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Detailed Information for Phage Lishka
Discovery Information
Isolation HostStreptomyces antibioticus UNT16F3
Found ByAnnelise Kemter
Year Found2024
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.20702 N, 97.14648 W Map
Discovery NotesI selected this spot as there were multiple bushes that appeared healthy, indicating richer soil. The soil was moist on top. I dug approximately 2 cm of top soil down, then I began my collection with a clean disposable spoon. I collected approximately 1/8 cup of soil.
Naming NotesLishka is just an assortment of random letters from my name rearranged to sound cool
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 8, 2025
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage5120
Genome length (bp)36221
Character of genome endsCircularly Permuted
GC Content72.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBC
SubclusterBC3
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusBeing Annotated (Expected completion by 5/1/2025)
Plaque NotesClear, circular in shape, .685mm average diameter
MorphotypeSiphoviridae
Number of Genes54
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 4x10^9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload