| Detailed Information for Phage Lishka | |
| Discovery Information | |
| Isolation Host | Streptomyces venezuelae ATCC 10712 |
| Found By | Annelise Kemter |
| Year Found | 2024 |
| Location Found | Denton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.20702 N, 97.14648 W Map |
| Discovery Notes | I selected this spot as there were multiple bushes that appeared healthy, indicating richer soil. The soil was moist on top. I dug approximately 2 cm of top soil down, then I began my collection with a clean disposable spoon. I collected approximately 1/8 cup of soil. |
| Naming Notes | Lishka is just an assortment of random letters from my name rearranged to sound cool |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 5120 |
| Genome length (bp) | 36221 |
| Character of genome ends | Circularly Permuted |
| GC Content | 72.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BC |
| Subcluster | BC3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, circular in shape, .685mm average diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 53 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLishka_1 Lishka_2 Lishka_3 Lishka_4 Lishka_5 Lishka_6 Lishka_7 Lishka_8 Lishka_9 Lishka_10 Lishka_11 Lishka_12 Lishka_13 Lishka_14 Lishka_15 Lishka_16 Lishka_17 Lishka_18 Lishka_19 Lishka_20 Lishka_21 Lishka_22 Lishka_23 Lishka_24 Lishka_25 Lishka_26 Lishka_27 Lishka_28 Lishka_29 Lishka_30 Lishka_31 Lishka_32 Lishka_33 Lishka_34 Lishka_35 Lishka_36 Lishka_37 Lishka_38 Lishka_39 Lishka_40 Lishka_41 Lishka_42 Lishka_43 Lishka_44 Lishka_45 Lishka_46 Lishka_47 Lishka_48 Lishka_49 Lishka_50 Lishka_51 Lishka_52 Lishka_53 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915841 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4x10^9 |
| Pitt Freezer Box# | 205 |
| Pitt Freezer Box Grid# | A10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
