Gordonia phage LittleRascal
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| Detailed Information for Phage LittleRascal | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Emre Nalbantoglu |
| Year Found | 2021 |
| Location Found | Fairfax, VA United States |
| Finding Institution | George Mason University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | -2°C |
| GPS Coordinates | 38.828637 N, 77.30683 W Map |
| Discovery Notes | This phage was collected on 01/29/2021 at 10:55 am in the gardens of Innovation Hall at George Mason University's Fairfax Campus. The garden topsoil sample collected was moist and a bit frozen. Soil was sandy, and no organic matter was present. Sample was collected about 2 inches below the surface. |
| Naming Notes | I named the phage LittleRascal because it was very challenging to purify. DNA isolation was also very trying. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | At first, while the phage was in the lytic phase, there was only one plaque morphology. Plaques were tiny and clear, and they had well-defined borders. Then, after the last round of purification, the phage began showing two plaque morphologies, one lytic and the other lysogenic. The lysogenic morphology was bigger and turbid. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 130 |
| Pitt Freezer Box Grid# | E2 |