Mycobacterium phage LittleTiny
LittleTiny Plaque Picture
LittleTiny EM Picture
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Detailed Information for Phage LittleTiny
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGanishah Charles-Estain
Year Found2016
Location FoundPhiladelphia, PA USA
Finding InstitutionUniversity of the Sciences (now St. Joseph's Univ)
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates39.944407 N, 75.209204 W Map
Discovery NotesSoil was mostly dry. Found in a floor pot in lab, that grew a little tree. The soil was filled with organic material;mostly dead foliage from the plant itself. Found at about 2.5 cm and kept at room temperature, 72 degrees Fahrenheit.
Naming NotesThe moment I saw Tiny, she was very difficult to see. She was much smaller than the plaques that I had isolated. After a series of full plate titers, her true morphology began to emerge. She was tiny and very turbid. In order to see her clearly, a light microscope was used to see her.
Then it hit me, TeenyTiny or Little Tiny?
I chose LittleTiny.
Sequencing Information
Sequencing Complete?No
Sequencing FacilityUniversity of Pittsburgh Genomics and Proteomics Core Laboratories
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe tail length is about 200 nm.
Diameter: 65 nm
DNA Concentration: 95.3 ng/ml
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.2 x 10^11
Date of SEA Lysate Titering Nov 10, 2016
Pitt Freezer Box# 34
Pitt Freezer Box Grid# E10
Available Files
Plaque PictureDownload
EM PictureDownload