Arthrobacter phage Lloydstein
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| Detailed Information for Phage Lloydstein | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis NRRL B-4425 |
| Found By | Camilo Harris |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | The soil sample was taken from moist soil below black mulch and pine needles in a hydrangea garden in a suburb of Ottawa (Barrhaven). The soil temperature could not be measured, but the ambient temperature was 13.6 degrees celsius. The soil sample itself was taken from a depth of around 5cm beneath the soil surface. Genomic DNA was sequenced on a ONT Minion, R10.4.1 chemistry using rapid barcoding V14 and PCR amplification. The longest read was 33,241 bp with 84% query cover, 88.95% identity to phage Bumble (cluster FH). Additional reads of 15,651bp had 94% query cover, 90.43% identity to phage Bumble (cluster FH). Further analysis in the lab showed that Lloydstein’s genome is almost identical to that of phage Circuit. However, the Circuit complete genome has not yet been uploaded to the NCBI database, so an exact percent identity cannot be confirmed from that site. This sequence appears to be incomplete since the longest contig is not within 10% of the completed phage circuit (cluster FH) sequence. |
| Naming Notes | The name “Lloydstein” was chosen as it is a family nickname for my mother. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Not tested for lysogeny as plaque assay showed clear plaque morphology - not characteristic of lysogenic phages. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | There are both large and small plaques present, the plaques are all very clear and there are many plaques spread out across the plate. |
| Morphotype | Myoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
