Mycobacterium phage Lodge
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| Detailed Information for Phage Lodge | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Camden Stewart |
| Year Found | 2022 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.988159 N, 86.445061 W Map |
| Discovery Notes | This phage was found after a rainy night in August. The plaques that this phage produced were very cloudy and turbid. They could be very are to see and distinguish at times. Throughout my research keeping this phage's population high tended to be difficult. Through my first 4 serial dilutions the population was relatively low after the first plate every time. Finally on the 5th serial dilution the population was finally high enough to proceed with my research. When isolating the DNA of this phage I was not able to get a very high concentration, but I was able to get a high enough concentration to put it through gel electrophoresis. When I ran the gel with our chosen restriction enzymes it was cut so many times that the DNA in the lanes look more like smears rather than cuts. |
| Naming Notes | The name of this phage comes from what we call the house I currently live in. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques of this particular phage are very cloudy and turbid and are about .2 cm in diameter. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 7.5x10^10 |
| Pitt Freezer Box# | 157 |
| Pitt Freezer Box Grid# | G4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
