Mycobacterium phage Lost
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| Detailed Information for Phage Lost | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emily Nordquist |
| Year Found | 2012 |
| Location Found | Baltimore, MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.326164 N, 76.616984 E Map |
| Discovery Notes | Found in an urban community garden bed, near a cement footpath. There were both live and decaying plants present. Fairly warm and humid day, slightly raining. Sample from about 4 cm below the surface. |
| Naming Notes | Towards the end of the process, both the DNA sample and the High Titer Lysate were lost and had to be remade, setting me back about a month. The phage is named Lost in honor of its long and troublesome history. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques are larger, with an average diameter of 2mm. They are cloudy and tend to blend together, but the individual plaques tend to have a round shape. The plaques can be different sizes, depending on the period of incubation. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | Lost |
| SEA Lysate Titer | 2.8x10^9 pfu/mL |
| Date of SEA Lysate Titering | Nov 29, 2012 |
| Pitt Freezer Box# | 13 |
| Pitt Freezer Box Grid# | C2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
