Microbacterium phage MADSci
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| Detailed Information for Phage MADSci | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Marisa DeCiucis |
| Year Found | 2020 |
| Location Found | New Haven, CT USA |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 41.334719 N, 72.941999 W Map |
| Discovery Notes | There were heavy rains a couple of days prior to collection as well as a tornado/tropical storm that passed through the area on 8/29/2020. The weather was sunny on the day of collection and the sample was taken from underneath decomposing plant material and debris from the recent storm. The soil had been recently disturbed from the addition of new debris from the storm. This was the second sample collected for the day. |
| Naming Notes | The name is a mixture of my initials and science. It also stands for "Mad Scientist". |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | This phage was tested for potential lysogens to confirm its lifecycle observed from its "pin-prick", lytic plaques. As previously observed, this phage is lytic because it did not form lysogens after performing a patch assay and homoimmunity test. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques look like "pin-pricks" and are .1 - .2mm in diameter. They are lytic because the edges are easily distinguishable. The average capsid width was 66.43 nm and the average capsid length was 68.19 nm. The average tail length was 152.79 nm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.25 e9 |
| Pitt Freezer Box# | 129 |
| Pitt Freezer Box Grid# | F5 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |