Mycobacterium phage Macula
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Detailed Information for Phage Macula
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByElizabeth Clemens
Year Found2011
Location FoundMissoula, MT USA
Finding InstitutionUniversity of Montana
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates46.861019 N, 113.989336 W Map
Discovery NotesThe sample was collected from a puddle on the corner of Daly Avenue nearest to the University of Montana campus in Missoula, Montana, at about 10:30 am on September 5th, 2011. The temperature on that day was approximately 82 degrees Fahrenheit (or 27.78 degrees Celsius). The puddle in question was fairly clean and clear looking despite a green algae growing throughout the bottom. The sample consisted of approximately two grams of moist dirt and sand taken from beneath about two inches of water. Being directly on a street corner, the water was constantly being disrupted. However, when this sample was taken, the sediment was not clouding in the water and had settled to the bottom of the puddle. At 10:30 am, sunlight was direct on the water, and there are no masses around to provide shade.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotation StatusNot sequenced
Plaque NotesIn general, the plaques are approximately 1/4 cm in diameter and faint and cloudy. There is some variation in size and shape: both the aforementioned plaques and pinprick looking plaques have been discovered on plates.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2011UMONmaculaEC
SEA Lysate Titer 2.5e8
Date of SEA Lysate Titering Nov 1, 2011
Pitt Freezer Box# 29
Pitt Freezer Box Grid# 70
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload