Arthrobacter phage Makima
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| Detailed Information for Phage Makima | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Victoria Hiscock |
| Year Found | 2025 |
| Location Found | Gibsonton, FL United States |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 27.811 N, 82.393 W Map |
| Discovery Notes | When discovered it was 86 degrees and cloudy in the area. The UV index was 5 and there was a small chance of precipitation in the surrounding areas. Makima came from soil that was near a small and shallow pond about a quarter mile into an unused hiking trail. |
| Naming Notes | Is named after powerful female character from Chainsaw man. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1155 |
| Genome length (bp) | 41592 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TTTACGGCAT |
| GC Content | 57.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FR |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | University of South Florida |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Plaques are lytic and mostly large (~2mm-3mm), clear, round, but can differ in size despite being clonal to form small or medium plaques when plated at a high density. |
| Morphotype | Siphoviridae |
| Number of Genes | 66 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMakima_1 Makima_2 Makima_3 Makima_4 Makima_5 Makima_6 Makima_7 Makima_8 Makima_9 Makima_10 Makima_11 Makima_12 Makima_13 Makima_14 Makima_15 Makima_16 Makima_17 Makima_18 Makima_19 Makima_20 Makima_21 Makima_22 Makima_23 Makima_24 Makima_25 Makima_26 Makima_27 Makima_28 Makima_29 Makima_30 Makima_31 Makima_32 Makima_33 Makima_34 Makima_35 Makima_36 Makima_37 Makima_38 Makima_39 Makima_40 Makima_41 Makima_42 Makima_43 Makima_44 Makima_45 Makima_46 Makima_47 Makima_48 Makima_49 Makima_50 Makima_51 Makima_52 Makima_53 Makima_54 Makima_55 Makima_56 Makima_57 Makima_58 Makima_59 Makima_60 Makima_61 Makima_62 Makima_63 Makima_64 Makima_65 Makima_66 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
