Mycobacterium phage Mattitude
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Detailed Information for Phage Mattitude
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKadence Kosanovich Johnson
Year Found2025
Location FoundBethlehem, PA United States
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature-7°C
GPS Coordinates40.60509 N, 75.37795 W Map
Discovery NotesThe soil sample was obtained outside the freshman dorm M&M on Lehigh University's campus. The ambient temperature was about -5ºC, so a very cold temperature. The soil was covered in snow and was wet / damp to the touch and dug up in large clumps that contained organic matter. The sample was collected at the surface of the ground.
Naming NotesMattitude was named after one of the Sturniolo Triplets (YouTubers), Matt, and the nickname he receives for his attitude. I have had some difficultly with the purification process of my phage, so I am giving it this name to reflect the attitude I am receiving from the phage.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Lysogeny NotesThe EM images of the filtered lysate sample revealed a hexagonal shaped head with a width of 54nm and a height of 75nm. The tails of the phages were long and flexible with a length of about 300nm, resulting in a head to tail ratio of 1:5.5. The lysate contained a high number of phages.
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaques contained a cloudy outer ring with a smaller dark center in a bullseye formation. The plaques were 0.4-0.6mm in size, with the a center of about 0.2mm.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.0 x 10^7 pfu/ml
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload