Mycobacterium phage Microphone
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| Detailed Information for Phage Microphone | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Junior Esteban De La Rosa Martinez |
| Year Found | 2022 |
| Location Found | Williamsburg, VA United States of America |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.271 N, 76.71634 W Map |
| Discovery Notes | Around 12:20 pm on 09/19/2022, I collected wet soil samples on 37.27100°N, 76.71634°W. I was interested in this location because there is a small water stream interacting with the ecosystem which I taught was a perfect location for phages to grow. Also, The soil of this area is exposed to a constant number of environmental factors because every day hundreds of students walk, ride their bicycles, and even skateboard to get to their classes faster. After collecting four spoons of soil from an idealistic location, I carried out a few protocols of enrichments, dilutions, amplifications, isolation, and an electron microscope grid to visualize and get my phage's DNA. |
| Naming Notes | I decided to call my phage Microphone because it has the peculiar shape of a microphone, an elongated body with a proportionally large head. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | University of Pittsburgh Genomics and Proteomics Core Laboratories |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Clear and small plaques with a little hallo were observed. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 160 |
| Pitt Freezer Box Grid# | F3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |