Mycobacterium phage MikesPholly
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| Detailed Information for Phage MikesPholly | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Michael Savoie |
| Year Found | 2017 |
| Location Found | Charlton, MA USA |
| Finding Institution | Worcester Polytechnic Institute |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.1 N, 71.9 E Map |
| Discovery Notes | The site of soil collection was at the edge of a flower bed that had lot of plant growth and previous soil amenities added. A garden trowel was used to remove the soil and it was deposited in a ziplock lock freezer bag and store at room temperature. Enrichment method was used to isolate the phage from the collected sample. The phage was purified and a phage lysate was collected. |
| Naming Notes | The name was inspired by my inability to capture a phage in my original sample. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques were very circular. They were opaque in appearance and very cloudy. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2X10^10 |
| Pitt Freezer Box# | 66 |
| Pitt Freezer Box Grid# | F2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
