| Detailed Information for Phage Mischief19 |
| Discovery Information |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Courtney Colson and Victoria Mahaney |
| Year Found | 2018 |
| Location Found | Catonsville, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 39.256184 N, 76.713126 W Map |
| Discovery Notes | soil was damp -- torrential rain proceeded in the days prior to collection. Air temperature was relatively humid, sample was collected in the morning. |
| Naming Notes | We were told to name our phage like a pet, so we named it like a pet. Mischief19 also lived up to its name during experimentation. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 31, 2019 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2711 |
| Genome length (bp) | 68430 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | n/a |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques have clear centers with turbid edges. Size of plaques are approximately 6 mm in diameter. Plaque development takes approximately 48 hours. |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK686071 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.8x10^10 pfu/mL |
| Pitt Freezer Box# |
83 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
