Microbacterium phage MisterLahey
MisterLahey Plaque Picture
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Detailed Information for Phage MisterLahey
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByGabriel Rogers
Year Found2019
Location FoundBowling Green, KY US
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.95654 N, 86.408447 W Map
Discovery NotesPhage was found in moist soil, approximately 1.5 inches under the surface. Soil was mainly composed of decaying organic matter such as leaves. Sample site was completely shaded at all hours of the day and located nearly 20 feet away from the edge of a creek. 6 serial dilutions were performed to isolate the phage, followed by the creation of a ten plate lysate. DNA isolation was performed to determine DNA concentration which was 89.2 ng/uL.
Naming NotesName originated from a character on a classic television show.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPlaques are clear, 1.25mm in diameter, and circular in shape.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.26x10^10 pfu/mL
Pitt Freezer Box# 100
Pitt Freezer Box Grid# E1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload