Streptomyces phage MountainPapa
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| Detailed Information for Phage MountainPapa | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Faith Davis, Mehrshad Devin |
| Year Found | 2021 |
| Location Found | Harpers Ferry, WV |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.325481 N, 77.740578 W Map |
| Discovery Notes | The soil MountainPapa was isolated from was collected 3 days after the most recent rainfall at 3 p.m., when it was 74 degrees Fahrenheit, right next to the Potomac River. The soil was damp. The phage was isolated from the soil 3 days after its collection. |
| Naming Notes | The phage was originally planned to be named MoutainMama as an homage to John Denver’s 1971 hit track, “Take Me Home, Country Roads.” MountainMama is a Microbacterium foliorum NRRL B-24224 phage isolated from Narrows, Virginia in 2019. MountainPapa was chosen as a replacement name that closely resembled the intended name. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | After a patch assay and a liquid phage release experiment, it was determined that MountainPapa can reproduce via the lysogenic lifecycle. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques grow at different rates. They begin to form about 9 hours after initial plating. At about 19 hours, half the plaques are full-sized and half are still growing. After 29 hours, all plaques reach the maximum size. They vary from 0.5 mm to 3 mm, but 3 mm is the maximum size. They have a clear center and a turbid halo surrounding them. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4 x 10^9 pfu/mL |
| Pitt Freezer Box# | 132 |
| Pitt Freezer Box Grid# | B4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
