Microbacterium phage MrGary
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Detailed Information for Phage MrGary
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByKarli Townsell, Allison Eudy
Year Found2021
Location FoundStatesville, NC United States
Finding InstitutionMitchell Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS CoordinatesUnavailable
Discovery Notes- Behind a house (in a backyard)
- Flat surface
- Dog feces in backyard, starting ~10 feet away from sample
- Surrounded by grass
- Dry
Naming NotesWe named Mr. Gary after Gary from the show SpongeBob SquarePants. Our phage's plaques on the full plate titer tests reminded us of Gary the snail's spots, so we felt drawn to name our phage "Mr. Gary."
Sequencing Information
Sequencing Complete?No
Sequencing FacilityPittsburgh Bacteriophage Institute
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque Notes- Initial plaques were abnormally small
- They were also spaced out because of low titer, and we had to complete many rounds of serial dilution to increase the titer and plate coverage.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 132
Pitt Freezer Box Grid# G5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload