Mycobacterium phage Munford
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| Detailed Information for Phage Munford | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sarah Stasel |
| Year Found | 2020 |
| Location Found | Munfordville, KY United States of America |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.357032 N, 86.017469 W Map |
| Discovery Notes | Phages replicated well and had visible plaques for first half of experimentation. Plaques became extremely turbid with a high titer once the 10 plate lysate was collected. During restriction enzyme digestion, only HaeIII made cuts in Munford phages. Other enzymes were not effective in cutting phage DNA. |
| Naming Notes | Named after the city where it was found and after my hometown. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques initially formed with bullseye morphology, there was variation in plaque diameter between 0.5-5.0 mm. Most common were above 2.5 mm in diameter. Plaques became fully turbid with diameter of ~2.5 mm. Phages were easily found with electron microscopy. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.3 x 10^13 pfu |
| Pitt Freezer Box# | 120 |
| Pitt Freezer Box Grid# | F1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
