Arthrobacter phage Nashara
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| Detailed Information for Phage Nashara | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis NRRL B-2880 |
| Found By | Professor Valenti |
| Year Found | 2025 |
| Location Found | Northport, NY United States of America |
| Finding Institution | SUNY Old Westbury |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 40.831068 N, 73.312934 W Map |
| Discovery Notes | Due to so much lysing in the beginning, we couldn’t get an exact titer right away. However, through multiple rounds of serial dilutions and adjustments to the concentration, we were able to get our lysate from our 10-7 webbed plate. Our titer was 8.2 x 10^10 pFU/mL. Through serial dilution and learning what concentration is needed in order to maintain a consistent lytic cycle, we were able to finally have a more usual or regular pattern to our phage growth across our plates. This is consistent with a lytic cycle, which refers to the stages & process of viral replication that results with the host cell’s death. |
| Naming Notes | We used the origin sample name, 1A Mulch, and used it in a different language. Nashara means mulch in Arabic. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | A head and long-like, flexible tail is kind of visible. This is known as a siphoviridae, a kind of phage morphology. Long tails = long “tape-measure gene”, which codes for a protein that determines the length of its tail. The size of our phages are semi- big & there are many clear circles, showing the visibly seen transparent border of the plaque within the bacterial lawn. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
