Mycobacterium phage Natan
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| Detailed Information for Phage Natan | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Elie Hachem |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | Soil samples were collected at Mer Bleue Bog, a protected environment, from the soil next to a tree, 2-3 inches deep in the ground. The soil was dark, wet, and rich in partially decomposed organic material. This area has a cool and consistently moist environment. These conditions are known to support microbial communities, which makes bog soils an interesting location for the isolation of bacteriophages. Genomic DNA was sequenced on an Oxford Nanopore Technologies MinION using R10.4.1 chemistry. The sequence assembled into one contig of 75,961 bp. BLASTn analysis showed 97% query coverage and 98.98% nucleotide identity to Mycobacterium phage Dumbo. Based on the high sequence similarity and the comparable genome size to related phages, this sequence likely represents a near-complete phage genome. |
| Naming Notes | I named my phage Natan. I got it from my friend's name Nathan, which I played on the word a little bit so it would be acceptable. This friend helped me a lot during my academic run, so I decided to name my phage after him. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques produced by phage Natan were medium in size. They showed a bullseye appearance. The plaques were not fully clear, which may reflect incomplete lysis. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
