Mycobacterium phage Negativitron
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| Detailed Information for Phage Negativitron | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Christopher Wojtas |
| Year Found | 2016 |
| Location Found | Stratford, CT USA |
| Finding Institution | Southern Connecticut State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 42°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | During isolation, the weather was overall constant with little to no rain over the past few days. It was collected during the night. Soil had no surrounding vegetation, mostly sand-like in its texture and mineral composition. |
| Naming Notes | This phage is named after Negativitron from LittleBigPlanet 2. Phages resemble infectious machines when infecting bacteria, hence the suffix (-tron). The name also correlates with "negating" bacteria. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques are consistently 0.5 or 1 mm in diameter, with small fuzzy edges, signifying a temperate phage present. Plaques are very subtle and indistinct. Due to its size, these plaques can make very intricate webbed plates. The final titer was 2.5 × 10^9 pfu/mL. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.5 x 10^9 |
| Date of SEA Lysate Titering | Dec 5, 2016 |
| Pitt Freezer Box# | 72 |
| Pitt Freezer Box Grid# | B1 |
| Available Files | |
| Plaque Picture | Download |