Mycobacterium phage Nemain
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Detailed Information for Phage Nemain
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNatasha George
Year Found2016
Location FoundSanta Cruz, CA USA
Finding InstitutionUniversity of California, Santa Cruz
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Discovery NotesSoil was collected from compost at the Chadwick Garden on the UCSC campus. Samples were then incubated with M.smegmatis for 1-2 days, and filtered media was spotted on a lawn of M. smegmatis to detect phage by plaque presence. Homogenous plaques were achieved through repeatedly picking plaques and plating dilutions with M. smegmatis.
Naming NotesNemain is a figure in Irish mythology associated with war and nightmare. In the Tain Bo Cuailnge, she shows up the night before the battle to sow terror and chaos in the sleeping army.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Cluster Life CycleTemperate
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesSmall pinhole-like plaques, that with enough time, develop translucent halos.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.6*10^11pfu/mL
Date of SEA Lysate Titering Nov 3, 2016
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload