Arthrobacter phage Nepean
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| Detailed Information for Phage Nepean | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis NRRL B-4425 |
| Found By | William Harris |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 45.27792 N, 75.76638 W Map |
| Discovery Notes | The phage was found in a soil sample taken from Mowat Farm Park in Ottawa, Canada, where a reforestation project was underway. Soil was collected by digging 3-5cm below the surface of long grasses, where organic matter was abundant. The ambient temperature was 21 degrees Celsius. Genomic DNA was sequenced on a ONT Minion, R10.4.1 chemistry using rapid barcoding V14 and PCR amplification. The longest read was 54,614bp with 78% query cover, 88.19% identity to phage ProfFrink (cluster AW). This sequence appears to be nearly complete based on its similarity to ProfFrink (AW) which is a similar length to the longest contig. |
| Naming Notes | The phage was named Nepean because it was discovered in Mowat Farm park, a park in the Nepean area of Ottawa. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The phage produces large and round plaques. The plaques are also slightly turbid. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |