Mycobacterium phage Norbaka
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Detailed Information for Phage Norbaka
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEllie Lunn and VyVy Moy
Year Found2023
Location FoundPeabody, MA United States of America
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS CoordinatesUnavailable
Discovery NotesThe sample was dug in the backyard of
a house in Peabody, MA. The sample was collected in front of the bush and between the two solar powered lights (Figure 1) The sample was collected by hand by turning a clean plastic
ziploc bag inside out, putting it over the hand and grabbing a handful of the dirt. The bag was kept between my hand and the soil the entire time, then inverted the bag back to its original
position while holding the sample still and sealed to keep the soil isolated. It was approximately 10:15 am on 9/19/23. It was around 70 degrees fahrenheit and sunny. The top of the soil was dry due to lack of rain, however the layers underneath where the sample was collected, about 6 inches down from the surface, were more moist as they were visually darker and softer to the
touch. The sample was collected right before lab that day and was stored in just the bag, with unknown temperature for storage, for about 2 hours until used in the lab.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesAll three plates were cloudy and had a cloudy ring around them. The 10-1 plate had some cloudy spots while the 10-3 plate had many spots that could be seen. From the plate we observed which was the 10-5 dilution plate, the phage we used was about 0.3 centimeters in diameter. There were at least 10 phages on this plate. All the phages and the plate itself were somewhat cloudy meaning that it was lysogenic. The plate appeared to be heterogeneous as some phages were different sizes than
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 182
Pitt Freezer Box Grid# C4
Available Files
Plaque PictureDownload