Mycobacterium phage Nymph
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| Detailed Information for Phage Nymph | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Callie Stempa |
| Year Found | 2022 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.83842 N, 86.4225 W Map |
| Discovery Notes | Due to Mycobacterium smegmatis being known to grow in freshwater environments and near bodies of water, the phage was collected from murky creek water. After enrichment, the phage underwent four serial dilutions. The plaque morphology appeared very consistent. After the one plate lysate was completed, the most countable plate resulted in a titer of 1.7 x 10^11 pfu/mL. A ten plate lysate was completed and resulted in a titer of 4.9 x 10^10 pfu/mL. Through electron microscopy, the tail was found to have an average length of 240.5 nm and an average capsid diameter of 68.5 nm. DNA isolation of the phage yielded a DNA concentration of 227.4 ng/uL. With the isolated DNA, restriction enzyme digests and gel electrophoresis was set up. The gel revealed that Nymph has a very GC rich genome. |
| Naming Notes | Nymph was chosen as its name to represent that the phage inhabits nature, specifically a river in this case. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | University of Pittsburgh Genomics and Proteomics Core Laboratories |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques were about 3-4 mm in diameter. The plaques were bull's eye plaques with a clear center and a turbid outside halo. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.9 x 10^10 pfu/mL |
| Pitt Freezer Box# | 157 |
| Pitt Freezer Box Grid# | G9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
