Mycobacterium phage OedipusRex
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Detailed Information for Phage OedipusRex | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Amanda E. Maldonado |
Year Found | 2017 |
Location Found | Baltimore, MD USA |
Finding Institution | Johns Hopkins University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 39.0 N, 76.0 W Map |
Discovery Notes | Phage collected from soil where tomoates were growing; some tomoates fell from branches onto the soil. Dug about two inches into the soil with a plastic spoon then scooped sample into testtube labelled: Sample O. Thin, small roots entwined in soil. Surroundings:29 degrees celsius, 15% precipitation, 49% humidity, 16 km/h wind, partly cloudy |
Naming Notes | Oedipus Rex is the name of an Athenian tragedy by Sophocles. It is the story of a man whose great high in life becomes a shattering low once a long hidden secret becomes revealed. Similarly, my phage isolation and purification process was going great, until a new phage morphology appeared, suggesting a contaminated lysate. This discovery led to additional weeks of purification in an effort to once again isolate my original phage, which was eventually accomplished. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | The plauqes are relatively small, about 0.15 cm in diamter. The EM revealed a long tail with a smaller head. Dilutions showed a consistency of small, round plaques. |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
Pitt Freezer Box# | 70 |
Pitt Freezer Box Grid# | H11 |
Available Files | |
Plaque Picture | Download |
EM Picture | Download |