Mycobacterium phage OliverOleOlea
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Detailed Information for Phage OliverOleOlea
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNathaniel Lee
Year Found2024
Location FoundLas Vegas, NV United States
Finding InstitutionUniversity of Nevada Las Vegas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates36.137778 N, 115.104167 W Map
Discovery NotesA soil sample was collected on September 3, 2024 in the front yard of a residential neighborhood at 7:20 am. The time of collection was deliberate in an attempt to protect the sample from UV and heat. The sample was collected at the base of an olive tree (olea europaea) in an attempt to maximize the possibility of successfully collecting phage containing bacteria. The sample collected was fine tightly compact dirt with relatively low moisture despite the sprinklers being run the night prior.
Naming NotesThe name Oliver was selected since the sample was collected from an olive tree, also because one of my dogs name is Oliver. Ole was chosen as a reference to to the chant people do at many sports games. Olea was also added to the name because it is the genus of the olive tree.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque Notesplaques typically display a 1.50 mm average, defined clearing zone surrounded by a well defined turbid halo zone indicating some lysogenic activity. The lysogenic activity seems to vary since some plaques have more defined haloing than others.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.66X10^10 PFU/μL
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload