Mycobacterium phage OliverWinston
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| Detailed Information for Phage OliverWinston | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Heath Crosby |
| Year Found | 2025 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.993369 N, 86.446256 W Map |
| Discovery Notes | My phage was collected at night from a soil sample 1-inch below the surface outside of the apartment in which I reside. The soil was still slightly moist due to a recent sprinkler cycle. 1X 7H9 Complete was used for enrichment purpose. Contamination was seen in my original spot-test, but was likely a result of my own improper aseptic technique in plating as no contamination was observed in a subsequent spot-test. Once my phage was isolated, I observed that the plaques it produced were large and somewhat turbid, signifying that I likely isolated a temperate phage. My calculated phage titer was 6x10^11. However, when sample was observed using electron microscopy, a low quantity of phage was observed. This may signify a phage with a high infection rate, which would explain the high PFU calculation. |
| Naming Notes | This phage was given the first and middle name of my dog, a little and annoying Cavachon. I felt the name fit perfectly. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques appeared turbid with bullseye morphology. They averaged approximately 5 mm in diameter once completely isolated. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 6.0x10^11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
