Mycobacterium phage OmegaMRC
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| Detailed Information for Phage OmegaMRC | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Team Plaque It Up |
| Year Found | 2017 |
| Location Found | Santa Monica , CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.093182 N, 118.587315 W Map |
| Discovery Notes | Phage OmegaMRC was isolated from soil, collected on 10/08/17 by O. Monifa Sawyerr at a depth of about 3 inches. This sample was a damp soil sample collected around a shrubby area in Santa Monica. There was a lot of plastic and cigarette butts in the area. There was also a lot of tree debris such as leaves and bark covering the area. Air temp at time of collection was 26.7˚C. OmegaMRC was isolated by direct plating at 35C. Characterization was performed by Team Plaque It Up (Carly Pierson, O. Monifa Sawyerr, Rina Watanabe). Midi-Lysate (Archive) Titer: 4.93*10^14 PFU/mL Date of High-titer Lysate Titering: 11/1/17 MIMG103AL-F17 Instructor: Dr. Amanda Freise Teaching Assistant: Andrew Lund Undergraduate Assistant: Keith Mitchell Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker |
| Naming Notes | We named our phage OmegaMRC because Omega means great and we wanted a connection of our phage to us, so we included our team's first initials. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaque morphology varies between halo and bullseye. The initial plaque picked was a bullseye plaque. From there, it has taken off and grown into a halo plaque Average size (and size range) diameter in mm: The average size is approx. 6mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 58 |
| Pitt Freezer Box Grid# | F5 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |