Mycobacterium phage Opalite
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| Detailed Information for Phage Opalite | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ava Carver |
| Year Found | 2025 |
| Location Found | Verona, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 38.8307 N, 84.68477 W Map |
| Discovery Notes | Sample Information Date collected: 9/1/2025 Location: Verona, Kentucky, United States Weather on the day the sample was collected: temperature 27.222°C, sunny, and minimal humidity. Physical Characteristics of Dirt Sample: -Very Dry -Crumbly texture(due to dryness) -Sample was collected an 1-2cm below surface. |
| Naming Notes | Opalite is a man-made gemstone that is translucent. This name was selected because the gemstone opalite signifies optimism for the future. In addition, its translucent characteristic aligns with this phage's turbid phenotype. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaque phenotypes: -Extremely turbid -Contained plaques of varying sizes Standard deviation and mean of plaque diameters were calculated on 10/14. Large Plaques (sample of 5) standard deviation: 0.1095cm mean: 0.42cm Small Plaques (sample of 5) standard deviation: 0.0837cm mean: 0.22cm On 10/14 there was a change in plaque morphology. Plaques observed were less turbid that previous plaques. This new phenotype remained consistent for the remainder of experiments. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1.89 10^9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
