Mycobacterium phage PVRamachandran
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| Detailed Information for Phage PVRamachandran | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Miles Thompson, Jack Maher |
| Year Found | 2019 |
| Location Found | West Lafayette, IN United States of America |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 25°C |
| GPS Coordinates | 40.432563 N, 86.932832 W Map |
| Discovery Notes | Physical Characteristics of Extraction Site: -Moist -Over 20m from nearest foot / car traffic -2-4 inches below the surface (under a 2 inch mat of mulch) -Temperature: 25 degrees C -Weather: Sunny, dry. Last rain 1 day ago. -Organic material: Bark / mulch, alpine currant roots -Sample was taken by the roots of an alpine currant plant at Purdue’s Horticulture Park. It was loamy with lots of bark particles. It was situated in an opening near a wooded area, not too far removed from the main campus. Since it was discovered in a mulch bed, this phage may have been transported from wherever the mulch was sourced rather than being endemic to its area of isolation. |
| Naming Notes | Named after the legendary Purdue chemistry professor, PVR. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Relatively small plaques. At high concentrations, they are less then a millimeter in diameter; at lower concentrations they tend to be larger (1-3 mm) with a higher proportion of halo to clear center. Temperate with halo of up to twice the radius of the interior clear circle. Slow growing. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.96 x 10^8 |
| Pitt Freezer Box# | 108 |
| Pitt Freezer Box Grid# | B9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |