Mycobacterium phage PVRamachandran
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Detailed Information for Phage PVRamachandran
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMiles Thompson, Jack Maher
Year Found2019
Location FoundWest Lafayette, IN United States of America
Finding InstitutionPurdue University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature25°C
GPS Coordinates40.432563 N, 86.932832 W Map
Discovery NotesPhysical Characteristics of Extraction Site:
-Moist
-Over 20m from nearest foot / car traffic
-2-4 inches below the surface (under a 2 inch mat of mulch)
-Temperature: 25 degrees C
-Weather: Sunny, dry. Last rain 1 day ago.
-Organic material: Bark / mulch, alpine currant roots
-Sample was taken by the roots of an alpine currant plant at Purdue’s Horticulture Park. It was loamy with lots of bark particles. It was situated in an opening near a wooded area, not too far removed from the main campus.

Since it was discovered in a mulch bed, this phage may have been transported from wherever the mulch was sourced rather than being endemic to its area of isolation.
Naming NotesNamed after the legendary Purdue chemistry professor, PVR.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesRelatively small plaques. At high concentrations, they are less then a millimeter in diameter; at lower concentrations they tend to be larger (1-3 mm) with a higher proportion of halo to clear center. Temperate with halo of up to twice the radius of the interior clear circle. Slow growing.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.96 x 10^8
Pitt Freezer Box# 108
Pitt Freezer Box Grid# B9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Virtual Digest PictureDownload
EM PictureDownload