Mycobacterium phage Pastuzo
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| Detailed Information for Phage Pastuzo | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Caroline Curtin |
| Year Found | 2022 |
| Location Found | Media, PA United States of America |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.917594 N, 75.405265 W Map |
| Discovery Notes | Pastuzo was found at the edge of a creek and foot bridge near the entrance to Middletown Township Memorial Park in Media, PA. The soil sample was collected by Dr. Lee-Soety on Monday, January 17, 2022 at 2:33 PM. The depth of the soil was 4 cm, and the soil conditions were wet. The ambient temperature was 2 °C with 77% humidity. The dew point was -2 °C, and the weather was overcast. The titer of the MTL is 6.7 x 10^10 pfu/mL. The titer of the HTL is 1.0 x 10^11 pfu/mL. |
| Naming Notes | Pastuzo was named for Paddington Bear's Uncle Pastuzo, who sacrificed his life (and his hat) to save the young bear. The plaques were large with a fuzzy halo, similar to the large stature and fuzzy nature of Uncle Pastuzo. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Pastuzo forms larger, clear plaques that are around 2 mm in diameter, with a thick, turbid halo surrounding each plaque. The plaques emerge after 24 to 48 hours in the 37 °C incubator. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 146 |
| Pitt Freezer Box Grid# | B5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |