Mycobacterium phage Pemberley
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| Detailed Information for Phage Pemberley | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Callie Freeman |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 24°C |
| GPS Coordinates | 36.985147 N, 86.455429 W Map |
| Discovery Notes | This sample was taken at approximately 10:00 PM on September 25, 2016 from soil surrounding a dormitory on the campus of Western Kentucky University. The soil was shaded, surrounded by several large trees and covered by the overhang of the dormitory's roof. The temperature of soil isolation was approximately 23.9 degrees Celsius. The soil was moist, grainy, dark in color and loose, but not muddy. While many trees and shrubbery surrounded the sample, waste such as cigarette stubs, food, and paper products were present as well. Plates were incubated at 30 degrees Celsius. Note: Because of a low DNA concentration and a problem with gel electrophoresis,I had to repeat DNA isolation. To complete this, I also had to create new lysate and obtain a new titer number. The titer I used for EM (9x10^9 pfu/ml) was from the initial lysate. However, the titer from the new lysate (2.2x10^9 pfu/ml) will be used in the restriction digest and gel electrophoresis. The titer date listed is of the first titer. The second titer was completed on 11-15-16. Note: I worked with a phage isolated by my classmate, Chloe Lindsey, as none of my own samples yielded plaques. |
| Naming Notes | The name Pemberley, Mr. Darcy's grand estate in Jane Austen's Pride and Prejudice, reflects my love of the Regency era, and the union of the humanities and science. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques are turbid "halos". The center of the plaque is slightly less turbid and clearly defined, while the outside of the plaque is more turbid and undefined, creating a "halo" appearance. Plaques range in size from approximately 2 mm - 2.5 mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2016WEKUpemberleyCF |
| SEA Lysate Titer | 9 x 10^9 pfu/mL |
| Date of SEA Lysate Titering | Oct 26, 2016 |
| Pitt Freezer Box# | 37 |
| Pitt Freezer Box Grid# | D2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
