| Detailed Information for Phage Pemberton |
| Discovery Information |
| Isolation Host | Gordonia terrae CAG3 |
| Found By | Hannah Harshaw and Madilyn Davis |
| Year Found | 2023 |
| Location Found | Charleston, AR United States of America |
| Finding Institution | Ouachita Baptist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 35.30165 N, 93.95316 W Map |
| Discovery Notes | Our phage was collected on 9-3-2023 at 4:45 p.m. It was found on our friend's cow farm at the pond that is on the bottom of the hill in the middle of the pasture. It was 95°F and 55% humid. It was collected from the surface level of the pond. The soil was moist and the land is always in the sun. |
| Naming Notes | It is named after our Honors OBU Connections professor, Dr. Barbara Pemberton, who we both adore! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 823 |
| Genome length (bp) | 64734 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCCGCGTAC |
| GC Content | 66.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CR |
| Subcluster | CR4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Ouachita Baptist University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | The plaques created from this phage lack turbidity and are very small. They are normally around 0.1cm in size. |
| Number of Genes | 93 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.6 X 10^8 |
| Pitt Freezer Box# |
183 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
