| Detailed Information for Phage PerroGrande22 |
| Discovery Information |
| Isolation Host | Rhodococcus equi NRRL B-16538 |
| Found By | Olivia Nuno |
| Year Found | 2025 |
| Location Found | Grand Rapids, MI United States |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.96994 N, 85.67436 W Map |
| Discovery Notes | Soil was found under a rock next to the Grand River in the amazing city of Grand Rapids. This rock was directly across the river from the DeVos Place. |
| Naming Notes | This phage is named after my stuffed animal Big Doggie, who has been with me since childhood. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 513 |
| Genome length (bp) | 79455 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 53.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CC |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Calvin University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Cloudy, small, smoothish edges. High concentration. |
| Morphotype | Siphoviridae |
| Number of Genes | 116 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
