| Detailed Information for Phage Phrappuccino |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sarah Mozdren |
| Year Found | 2015 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.787500 N, 86.101600 W Map |
| Discovery Notes | Phage was isolated by our modified direct plating procedure. The soil was moist. Soil came from Dr. Joseph Stukey's compost, in which it was well mixed frequently. A straight forward depth of collection is not relevant. This sample was collected on August 31st, 2015 at about 7:00 PM |
| Naming Notes | I really enjoy coffee, especially for college, so this name is very appropriate for me. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 19, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 397 |
| Genome length (bp) | 136361 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are very tiny, averaging in 1-2mm in diameter. The growth of these plaques takes up to 48 hours instead of the average 24. |
| Morphotype | Myoviridae |
| Number of Genes | 201 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK937592 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.35x10^10 |
| Pitt Freezer Box# |
19 |
| Pitt Freezer Box Grid# |
E4 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
