Mycobacterium phage Pika17
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Detailed Information for Phage Pika17
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCaroline Kominick
Year Found2021
Location FoundPhiladelphia, PA United States
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature4°C
GPS Coordinates40.245 N, 75.151 W Map
Discovery NotesPika17 was found right beside a tree next to the parking lot and Schuylkill River at Flat Rock Park on January 25th, 2021. The weather was extremely cold with high winds and dry air. The humidity was 50% and the ambient temperature was 4.44 degrees C. The depth of soil collection was 12 cm. Additionally, the HTL was 1.4 x 10^11 pfu/mL. The DNA concentration was 856.9 ng/uL. The average capsid size was 47.5 nm in diameter and the average tail size was 250 nm in length. This was calculated from a sample size of 8.
Naming NotesPika is short for the Pokemon Pikachu. Pikachu's long tail reminded me of the siphoviridae phage tails. There was already a phage with the name Pikachu, so Pika was created. 17 is a reference to my birthday.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe phage produced clear, very small plaques with rounded edges. The plaques were about 1 mm in diameter and appeared after 24 hrs of incubation at 37 degrees C.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload