Mycobacterium phage Pika17
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| Detailed Information for Phage Pika17 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Caroline Kominick |
| Year Found | 2021 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 4°C |
| GPS Coordinates | 75.151 N, 40.245 W Map |
| Discovery Notes | Pika17 was found right beside a tree next to the parking lot and Schuylkill River at Flat Rock Park on January 25th, 2021. The weather was extremely cold with high winds and dry air. The humidity was 50% and the ambient temperature was 4.44 degrees C. The depth of soil collection was 12 cm. Additionally, the HTL was 1.4 x 10^11 pfu/mL. The DNA concentration was 856.9 ng/uL. The average capsid size was 47.5 nm in diameter and the average tail size was 250 nm in length. This was calculated from a sample size of 8. |
| Naming Notes | Pika is short for the Pokemon Pikachu. Pikachu's long tail reminded me of the siphoviridae phage tails. There was already a phage with the name Pikachu, so Pika was created. 17 is a reference to my birthday. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The phage produced clear, very small plaques with rounded edges. The plaques were about 1 mm in diameter and appeared after 24 hrs of incubation at 37 degrees C. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 130 |
| Pitt Freezer Box Grid# | A1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |