Microbacterium phage Pitachip
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| Detailed Information for Phage Pitachip | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Laura Ragan Gay and Andrew Hinton |
| Year Found | 2019 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.992778 N, 86.4175 W Map |
| Discovery Notes | Found in a park near recreation buildings. Serial dilutions were performed three times, with the last one being a big and small test. A one-plate lysate was then created, afterward, a ten plate lysate was created with a final titer of 5.8*10^9.DNA isolation was performed with a final concentration of 148.3 μg/mL. A restriction enzyme digest was run on a gel with ten enzymes. |
| Naming Notes | Named after my favorite snack, Pitachips and dip. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | clear plaques Varied between 0.2 mm and 0.4mm, however big and small test confirmed they were coming from the same phage population. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.8*10^9 |
| Pitt Freezer Box# | 100 |
| Pitt Freezer Box Grid# | E5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
