Mycobacterium phage PrincessMocha
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| Detailed Information for Phage PrincessMocha | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sanam Krishnani |
| Year Found | 2024 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.97815 N, 86.41795 W Map |
| Discovery Notes | The sample was collected in Bowling Green, KY. It was collected from potting soil that is watered regularly. The soil is a mixture of peat moss, vermiculite, perlite, and other organic material. It has a high level of organic matter to provide nutrients to plants. The temperature upon collection was 73 degrees F, and it was moderately cloudy. |
| Naming Notes | I named this after my nickname my mom gave me and my dog name. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | No lysogens present. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Throughout the purifying process, the plaques of PrincessMocha were turbid on each of the plates that were used to continue this process. All with an organic circle shape. For this reason, four serial dilutions were performed to ensure the phage was purified before a high-titer lysate was created. The diameter is about 0.5 - 1 mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 5 * 10^8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
