Mycobacterium phage Problematic
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| Detailed Information for Phage Problematic | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Miranda Raevsky |
| Year Found | 2012 |
| Location Found | Canton, MO USA |
| Finding Institution | Culver-Stockton College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.128635 N, 91.530101 W Map |
| Discovery Notes | The sample the phage was collected from was gathered on 9/6/2012 at approximately 9:20 a.m. on the Culver-Stockton College campus. The outside temperature was 85 degrees Fahrenheit. The sample was collected approximately 4 centimeters below the surface from a moist soil close to a tree. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques for this phage went through cycles of changing sizes even after purification. The plaques were initially fairly large but after about two weeks they began to appear as small plaques. The cycle went back and forth between big and small approximately every two weeks. The plaques always appeared to be lytic phages. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 13.4 x 10^10 pfu/mL |
| Date of SEA Lysate Titering | Nov 5, 2012 |
| Pitt Freezer Box# | 43 |
| Pitt Freezer Box Grid# | 24 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
