Mycobacterium phage Pupil
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| Detailed Information for Phage Pupil | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kimberly Paquette |
| Year Found | 2017 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 32°C |
| GPS Coordinates | 42.7803 N, 86.1993 W Map |
| Discovery Notes | Pupil was isolated from soil found at the Holland State Park on August 24, 2017 at 7:45pm. The soil was collected from an isolated area with fallen leaves from the surrounding trees and was about 1 inch below the surface. This area was shady and the outside temperature was 65°F with 56% humidity. |
| Naming Notes | The name "Pupil" comes from the phage's plaque morphology. The morphology has a slight turbid ring around a circular center, which resembles the pupil in the eye. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Pupil has a diameter of 0.5 millimeters and is a turbid plaque. Some plaques have a faint turbid ring around a circular center while other plaques do not have the ring. Plaques appear best after 48 hours in a 32°C incubator. Pupil does not grow in a 42°C incubator and different infection times do not produce a significant difference in plaque morphology. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 50 |
| Pitt Freezer Box Grid# | E4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
