Mycobacterium phage Pupil
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Detailed Information for Phage Pupil
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKimberly Paquette
Year Found2017
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature32°C
GPS Coordinates42.7803 N, 86.1993 W Map
Discovery NotesPupil was isolated from soil found at the Holland State Park on August 24, 2017 at 7:45pm. The soil was collected from an isolated area with fallen leaves from the surrounding trees and was about 1 inch below the surface. This area was shady and the outside temperature was 65°F with 56% humidity.
Naming NotesThe name "Pupil" comes from the phage's plaque morphology. The morphology has a slight turbid ring around a circular center, which resembles the pupil in the eye.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPupil has a diameter of 0.5 millimeters and is a turbid plaque. Some plaques have a faint turbid ring around a circular center while other plaques do not have the ring. Plaques appear best after 48 hours in a 32°C incubator. Pupil does not grow in a 42°C incubator and different infection times do not produce a significant difference in plaque morphology.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 50
Pitt Freezer Box Grid# E4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload