Gordonia phage Purkinje
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Detailed Information for Phage Purkinje
Discovery Information
Isolation HostGordonia terrae 3612
Found ByConnor Scanlon
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature30°C
GPS Coordinates40.445556 N, 79.962778 W Map
Discovery Notes​Soil sample was collected roughly two centimeters below the surface of the ground. There were no stray pieces of grass in the sample, however one rock was identified and separated from the sample prior to its integration with phage buffer.
Purkinje is a siphoviridae bacteriophage featuring a icosahedral capsid with a diameter of roughly 50 nm. The tail of Purkinje is roughly 300 nm long.
Based upon a restriction digest and subsequent gel electrophoresis of Purkinje DNA, evidence supports the conclusion that Purkinje is a novel Gordonia terrae bacteriophage, as there is little overlap in present bands.
Naming NotesThe name of this phage is based upon the branching GABAergic Purkinje cells found within the cerebellum of the human brain.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPlaques are clear in complexion, suggesting that Purkinje is lytic in nature. Plaques are moderate in size, with a diameter of roughly half a millimeter.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 90
Pitt Freezer Box Grid# H12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload