Mycobacterium phage Rayquaza
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Detailed Information for Phage Rayquaza | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Logan Robinson |
Year Found | 2024 |
Location Found | Bowling Green, KY United States of America |
Finding Institution | Western Kentucky University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 36.98679 N, 86.44861 W Map |
Discovery Notes | This phage was derived from a sample that was taken from the base of the tallest tree on WKU's campus. The plaque is lysogenic, shown by its turbid morphology. The sample was taken from dry soil, as the area had gone through a dry spell at the time of collection. The phage was isolated through and enriched sample and a series of serial dilutions. |
Naming Notes | The name is the name of my favorite videogame character from when I was a kid, it resemples a long, spindly dragon with a head bigger than it's body, so I thought it fit to name my phage after it. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Lysogeny Notes | After viewing the turbid plaques, the lysogens were plated and Rayquaza was spotted in 6 degrees of ten on the plate. There were no plaques presenting on the plate, and the control was positve for phage, thus is is concluded that Rayquayza phage's lysogens have immunity to themselves. |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | The average size of single plaque is 0.5 cm, with the plaques being turbid, and having a small halo the very center of the plaque. The phage is of the temperate lifestyle, as shown by the turbid morphology. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Not in Pitt Archives |
SEA Lysate Titer | 2.27 * 10^9 pfu / mL |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |