Mycobacterium phage Reesesthecat
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Detailed Information for Phage Reesesthecat
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJake Oliver & Joshua Cohen
Year Found2024
Location FoundBoulder, CO United States of America
Finding InstitutionUniversity of Colorado Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.00887 N, 105.269349 W Map
Discovery NotesPhage isolated from enriched soil sample in a very damp, shaded area under a bush. The sample was taken at 10:25 AM next to a laboratory building on CU Boulder's campus. Some muddy and some dry soil included in the sample.
Naming NotesOne of Jake's cats (a tortoiseshell) is named Reeses for her resemblence of a Reeses Peanut Butter Cup. Since Reeses is one of the two cutest cats in the world, she deserves to be enshrined in scientific history.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaques have internal transparent clearings surrounded by a turbid halo. The total plaque diameter is about 5 mm; the halo is about 1.2 mm in width; and the internal clearing is about 2 mm in diameter. Halo turbidity increases when plaques occur adjacently, resulting in the appearence of the adjacent plaques being more oblong that those that occur alone. Critically, the internal transparent clearings remain clear and circular. At higher titers, the plaques may appear smaller, and the halo may be less obvious, though they should be uniform.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2.8(10)^10 PFU mL^-1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload