Gordonia phage RemJ
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| Detailed Information for Phage RemJ | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Kailee Lear |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4472 N, 79.9529 W Map |
| Discovery Notes | Collection location & time: Ruskin Hall courtyard, 5:35 p.m. Collection conditions: soil -- fertile, damp, compact, under mulch, not great access to sunlight (buildings surrounding location and tree over top) weather -- hot, 93 degrees F, sunny (though in shaded area), 43% humidity, no rain for the past several days vegetation -- grass, moss, bushes, one tree Transport time: 7 minutes (collection location to storage location) & 1 hour, 50 minutes (storage location to lab) Storage conditions: room temperature desk drawer Storage time: 5:42 pm (9/4) to 2:45 (9/5) |
| Naming Notes | Named after my dog Remi: RemJ = Remi Junior |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | A lysogen test was attempted, however the resulting plate after serial dilutions spotted onto a spot test showed plaques on the phage buffer square. Therefore, results are ambiguous: difficult to tell if plaques on the plate are due to the potential source of phage in the buffer or due to my own lysate. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | small -- approximately 1 mm circular clear -- indicating lytic phage no halos |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1x10^9 pfu/mL |
| Pitt Freezer Box# | 91 |
| Pitt Freezer Box Grid# | A6 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |