| Detailed Information for Phage RemusLoopin | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Shirin Ludwig and Amanda Saikali |
| Year Found | 2018 |
| Location Found | Annapolis, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 38.982 N, 76.4915 W Map |
| Discovery Notes | Ambient temperature was 76°F; soil was dry and filled with organic matter; collected approx. 1 inch below the surface. |
| Naming Notes | The name RemusLoopin was inspired by the clear "loops" of the plaque morphology, along with our love for Harry Potter :) |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2019 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 4245 |
| Genome length (bp) | 41376 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCCGGCCCCA |
| GC Content | 62.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BB |
| Subcluster | BB2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | RemusLoopin is likely a virulent phage despite plaque appearance, as attempts to isolate lysogens were unsuccessful. |
| Other Cluster Members | |
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are circular and vary in size; measure 1-4.5mm in diameter. Display turbid centers and turbid edges, separated by distinct rings of clarity in a "bullseye" pattern. |
| Morphotype | Siphoviridae |
| Number of Genes | 56 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewRemusLoopin_1 RemusLoopin_2 RemusLoopin_3 RemusLoopin_4 RemusLoopin_5 RemusLoopin_6 RemusLoopin_7 RemusLoopin_8 RemusLoopin_9 RemusLoopin_10 RemusLoopin_11 RemusLoopin_12 RemusLoopin_13 RemusLoopin_14 RemusLoopin_15 RemusLoopin_16 RemusLoopin_17 RemusLoopin_18 RemusLoopin_19 RemusLoopin_20 RemusLoopin_21 RemusLoopin_22 RemusLoopin_23 RemusLoopin_24 RemusLoopin_25 RemusLoopin_26 RemusLoopin_27 RemusLoopin_28 RemusLoopin_29 RemusLoopin_30 RemusLoopin_31 RemusLoopin_32 RemusLoopin_33 RemusLoopin_34 RemusLoopin_35 RemusLoopin_36 RemusLoopin_37 RemusLoopin_38 RemusLoopin_39 RemusLoopin_40 RemusLoopin_41 RemusLoopin_42 RemusLoopin_43 RemusLoopin_44 RemusLoopin_45 RemusLoopin_46 RemusLoopin_47 RemusLoopin_48 RemusLoopin_49 RemusLoopin_50 RemusLoopin_51 RemusLoopin_52 RemusLoopin_53 RemusLoopin_54 RemusLoopin_55 RemusLoopin_56 — tRNA RemusLoopin_57 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK686068 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.6x10^10 pfu/mL |
| Pitt Freezer Box# | 83 |
| Pitt Freezer Box Grid# | H10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
