Mycobacterium phage Ringtails15
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| Detailed Information for Phage Ringtails15 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | LyticLemur |
| Found By | Lindsay Wright |
| Year Found | 2015 |
| Location Found | Cullowhee, NC USA |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.311666 N, 83.174722 W Map |
| Discovery Notes | My phage was found on August 21, 2015. The sample was dark brown crumbly soil, collected approximately two and a half centimeters deep, in a non-populated area close to the McKee building on Western Carolina’s campus. The temperature was approximately 24.4°C and the GPS coordinates of the location is 35° 18’ 42” N 83° 10’ 49” W. One neat thing about my sample was that after it was centrifuged there was a small worm in my sample. My plaques were tested by both enrichment and direct plating. Refer to “Direct Plating/Phage Enrichment” protocol to see the process of both. I spot tested three spots from my direct plating and three spots from my enrichment. After my spots were tested one of them appeared positive for phage. My positive spot originated from my enrichment 100 plate. The plaque that appeared positive was small and clear, so it appeared to be lytic. I had discovered phage in my first soil collection. |
| Naming Notes | I named my phage the Lytic Lemur because on the EM images that were taken, the long tail with protein rings appeared similar to those of a ring-tailed lemur. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Pen point in size .0661 micrometer average capsid .350 micrometer average tail Lytic 24-48hrs growing time |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.0x10^10 |
| Date of SEA Lysate Titering | Sep 25, 2015 |
| Pitt Freezer Box# | 19 |
| Pitt Freezer Box Grid# | G10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |