Arthrobacter phage RoxyNova
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| Detailed Information for Phage RoxyNova | |
| Discovery Information | |
| Isolation Host | Arthrobacter atrocyaneus NRRL B-2883 |
| Found By | Naomi Hammad |
| Year Found | 2024 |
| Location Found | Lisle, IL United States |
| Finding Institution | Benedictine University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.77 N, 88.09 W Map |
| Discovery Notes | I acquired my soil from the Benedictine parking lot in Lisle, IL. The night before I collected the soil, there had been rain, making the soil moist. The soil was still particularly wet but hardened and turned grainy the further I dug. It was collected at 11:15 AM. I used a shovel and gloves, reaching about 1-2 inches before extracting approximately ½ pound of soil. I chose this specific location because the soil was said to be rich with phages. The soil sample was dark and moist, containing small pieces of rocks, and plant stems, and had a grainy texture. |
| Naming Notes | I chose the name RoxyNova because my cat's name is Roxy. Nova was chosen as a part of this name since nova is derived from "novus," meaning "new," for my newly discovered phage. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaque diameter: 4mm Plaque radius: 2mm Phage RoxyNova formed clear lytic plaques with a diameter of 4mm and a radius of 2mm. Since my radius was larger, I needed fewer plaques to generate a webbed plate. The temperature the phage grew the best at 37°C. This tells us that the phage amplifies better at higher temperatures rather than lower ones. Phage RoxyNova is from the Siphoviridae family. The capsid of my phage is attached to a tail. Phages from the Siphoviridae family have long, flexible tails that are thin and non-contractile. Siphoviridae phages also have double-stranded DNA genomes. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
